Bat Severe Acute Respiratory Syndrome-Like Coronavirus WIV1 Encodes an Extra Accessory Protein, ORFX, Involved in Modulation of the Host Immune Response.
Identifieur interne : 001074 ( Main/Exploration ); précédent : 001073; suivant : 001075Bat Severe Acute Respiratory Syndrome-Like Coronavirus WIV1 Encodes an Extra Accessory Protein, ORFX, Involved in Modulation of the Host Immune Response.
Auteurs : Lei-Ping Zeng [République populaire de Chine] ; Yu-Tao Gao [République populaire de Chine] ; Xing-Yi Ge [République populaire de Chine] ; Qian Zhang [République populaire de Chine] ; Cheng Peng [République populaire de Chine] ; Xing-Lou Yang [République populaire de Chine] ; Bing Tan [République populaire de Chine] ; Jing Chen [République populaire de Chine] ; Aleksei A. Chmura [États-Unis] ; Peter Daszak [États-Unis] ; Zheng-Li Shi [République populaire de Chine]Source :
- Journal of virology [ 1098-5514 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Antiviraux (pharmacologie), Cadres ouverts de lecture (génétique), Cadres ouverts de lecture (immunologie), Cellules HeLa, Cellules Vero, Chiroptera (virologie), Facteur de transcription NF-kappa B (métabolisme), Humains, Interféron bêta (pharmacologie), Réplication virale (immunologie), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (traitement médicamenteux), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (immunologie).
- MESH :
- génétique : Cadres ouverts de lecture.
- immunologie : Cadres ouverts de lecture, Réplication virale, Syndrome respiratoire aigu sévère, Virus du SRAS.
- métabolisme : Facteur de transcription NF-kappa B.
- pharmacologie : Antiviraux, Interféron bêta.
- traitement médicamenteux : Syndrome respiratoire aigu sévère.
- virologie : Chiroptera, Syndrome respiratoire aigu sévère.
- Animaux, Cellules HeLa, Cellules Vero, Humains.
English descriptors
- KwdEn :
- Animals, Antiviral Agents (pharmacology), Chiroptera (virology), Chlorocebus aethiops, HeLa Cells, Humans, Interferon-beta (pharmacology), NF-kappa B (metabolism), Open Reading Frames (genetics), Open Reading Frames (immunology), SARS Virus (immunology), Severe Acute Respiratory Syndrome (drug therapy), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Vero Cells, Virus Replication (immunology).
- MESH :
- chemical , metabolism : NF-kappa B.
- chemical , pharmacology : Antiviral Agents, Interferon-beta.
- drug therapy : Severe Acute Respiratory Syndrome.
- genetics : Open Reading Frames.
- immunology : Open Reading Frames, SARS Virus, Severe Acute Respiratory Syndrome, Virus Replication.
- virology : Chiroptera, Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, HeLa Cells, Humans, Vero Cells.
Abstract
Bats harbor severe acute respiratory syndrome (SARS)-like coronaviruses (SL-CoVs) from which the causative agent of the 2002-2003 SARS pandemic is thought to have originated. However, despite the fact that a large number of genetically diverse SL-CoV sequences have been detected in bats, only two strains (named WIV1 and WIV16) have been successfully cultured in vitro These two strains differ from SARS-CoV only in containing an extra open reading frame (ORF) (named ORFX), between ORF6 and ORF7, which has no homology to any known protein sequences. In this study, we constructed a full-length cDNA clone of SL-CoV WIV1 (rWIV1), an ORFX deletion mutant (rWIV1-ΔX), and a green fluorescent protein (GFP)-expressing mutant (rWIV1-GFP-ΔX). Northern blotting and fluorescence microscopy indicate that ORFX was expressed during WIV1 infection. A virus infection assay showed that rWIV1-ΔX replicated as efficiently as rWIV1 in Vero E6, Calu-3, and HeLa-hACE2 cells. Further study showed that ORFX could inhibit interferon production and activate NF-κB. Our results demonstrate for the first time that the unique ORFX in the WIV1 strain is a functional gene involving modulation of the host immune response but is not essential for in vitro viral replication.
DOI: 10.1128/JVI.03079-15
PubMed: 27170748
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Bats harbor severe acute respiratory syndrome (SARS)-like coronaviruses (SL-CoVs) from which the causative agent of the 2002-2003 SARS pandemic is thought to have originated. However, despite the fact that a large number of genetically diverse SL-CoV sequences have been detected in bats, only two strains (named WIV1 and WIV16) have been successfully cultured in vitro These two strains differ from SARS-CoV only in containing an extra open reading frame (ORF) (named ORFX), between ORF6 and ORF7, which has no homology to any known protein sequences. In this study, we constructed a full-length cDNA clone of SL-CoV WIV1 (rWIV1), an ORFX deletion mutant (rWIV1-ΔX), and a green fluorescent protein (GFP)-expressing mutant (rWIV1-GFP-ΔX). Northern blotting and fluorescence microscopy indicate that ORFX was expressed during WIV1 infection. A virus infection assay showed that rWIV1-ΔX replicated as efficiently as rWIV1 in Vero E6, Calu-3, and HeLa-hACE2 cells. Further study showed that ORFX could inhibit interferon production and activate NF-κB. Our results demonstrate for the first time that the unique ORFX in the WIV1 strain is a functional gene involving modulation of the host immune response but is not essential for in vitro viral replication.</div>
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<tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Zeng, Lei Ping" sort="Zeng, Lei Ping" uniqKey="Zeng L" first="Lei-Ping" last="Zeng">Lei-Ping Zeng</name>
</region>
<name sortKey="Chen, Jing" sort="Chen, Jing" uniqKey="Chen J" first="Jing" last="Chen">Jing Chen</name>
<name sortKey="Gao, Yu Tao" sort="Gao, Yu Tao" uniqKey="Gao Y" first="Yu-Tao" last="Gao">Yu-Tao Gao</name>
<name sortKey="Ge, Xing Yi" sort="Ge, Xing Yi" uniqKey="Ge X" first="Xing-Yi" last="Ge">Xing-Yi Ge</name>
<name sortKey="Peng, Cheng" sort="Peng, Cheng" uniqKey="Peng C" first="Cheng" last="Peng">Cheng Peng</name>
<name sortKey="Shi, Zheng Li" sort="Shi, Zheng Li" uniqKey="Shi Z" first="Zheng-Li" last="Shi">Zheng-Li Shi</name>
<name sortKey="Tan, Bing" sort="Tan, Bing" uniqKey="Tan B" first="Bing" last="Tan">Bing Tan</name>
<name sortKey="Yang, Xing Lou" sort="Yang, Xing Lou" uniqKey="Yang X" first="Xing-Lou" last="Yang">Xing-Lou Yang</name>
<name sortKey="Zhang, Qian" sort="Zhang, Qian" uniqKey="Zhang Q" first="Qian" last="Zhang">Qian Zhang</name>
</country>
<country name="États-Unis"><region name="État de New York"><name sortKey="Chmura, Aleksei A" sort="Chmura, Aleksei A" uniqKey="Chmura A" first="Aleksei A" last="Chmura">Aleksei A. Chmura</name>
</region>
<name sortKey="Daszak, Peter" sort="Daszak, Peter" uniqKey="Daszak P" first="Peter" last="Daszak">Peter Daszak</name>
</country>
</tree>
</affiliations>
</record>
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